Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaReference or source
S. mutans strains
 T8Wild type, Mut+21
 CBM0Tcr ΔmutA Mut, expression host for mutacin II variantsThis study
S. sobrinus OMZ176Mutacin-sensitive indicator4
Plasmids
 pUC19AprlacZα29
 pCRIIApr Kmr, cloning vectorInvitrogen
 pNoTA/T7Apr, cloning vector5′→3′ Inc.
 pCBM1Apr Kmr, pCRII with 0.75-kb PCR fragment containing mutA downstream regionThis study
 pCBM2Apr Kmr, pCRII with 1.3-kb mut3 SSP-PCR fragment containing mutAupstream regionThis study
 pCBM3AprKmr, pCRII with both mutA upstream and downstream regionsThis study
 pCBM4AprKmr Tcr, pCBM3 with tetM gene inserted at HpaI site in the mutA upstream regionThis study
 pCBM5Apr, pNoTA/T7 with 2.2-kb mut2 SSP-PCR fragment containing complete mutA coding regionThis study
 pCBM6Apr Kmr, pCBM5 with aphIII gene inserted at HpaI site in the mutA upstream region, used for site-directed mutagenesisThis study
 V7AApr Kmr, analog of pCBM6, confers production of V7A mutacinThis study
 P9AApr Kmr, analog of pCBM6, confers production of P9A mutacinThis study
 T10AAprKmr, analog of pCBM6, confers production of T10A mutacinThis study
 T10SApr Kmr, analog of pCBM6, confers production of T10S mutacinThis study
 C15AApr Kmr, analog of pCBM6, confers production of C15A mutacinThis study
 C26AAprKmr, analog of pCBM6, confers production of C26A mutacinThis study
 C27AApr Kmr, analog of pCBM6, confers production of C27A mutacinThis study
 ΔN1Apr Kmr, analog of pCBM6, confers production of ΔN1 mutacinThis study
  • a Apr, ampicillin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance; Mut, mutacin negative; Mut+, mutacin positive.