Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
P. putida strains
 CMC4(pWW0), Kmr3MB+ mini-Tn5/ Km::(xylSPm::lacIPlac::gef)This study
 CMC5Kmr, pCC102, mini-Tn5/ Tc::Pm::′luxAB This study
 EEZ32(pWW0), pCC102, Kmr Tcr3MB+ 26
 KT2440Prototroph, Cmr 8
 UWC1Rifr derivative of KT2440 23
E. coli strains
 CC118λpir Rifr, host for pSM1350 9
 HB101Smr, host for pRK600 13
 pCC102Kmr, xylS2Pm::lacI 5
 pRK600Cmr, ColE1 oriV, RK2mob+tra+ 13
 pSM1350Kmr, mini-Tn5, xylSPm::lacI, Plac::gef This study
 pWW0IncP9, mob+ tra+ 3MB+ 21
  • a Kmr, Apr, Cmr, and Rifr, resistance to kanamycin, ampicillin, chloramphenicol, and rifampin, respectively; 3MB+, ability to grow on 3-methylbenzoate.