Table 3.

Characterization of mutacin II variants

Mutacin IICalculated mass (Da)Determined mass (Da)aBiological activityb
Wild type3,2453,245.4 ± 0.6+d
ΔN13,131NDc
V7A3,2173,217.8 ± 0.1+
P9A3,2193,218.4 ± 1.2±
T10A3,233ND
T10S3,2303,230.4 ± 1.1+
C15A3,2133,212.4 ± 1.2
C26A3,2133,214.2 ± 0.7
C27A3,2133,212.8 ± 1.4±
  • a Mass of purified extracellular product.

  • b As determined by a deferred antagonism assay (stabs).

  • c ND, not detected.

  • d +, activity; −, no activity; ±, low level of activity (less than 10% of the wild-type activity).