Table 1.

Total DAPI cell counts and relative percentages of hybridized cells with specific probes

Samples and depth (cm)Absolute DAPI counts (cells/cm3 [108]) (mean ± SD)% of cells hybridized with probea:% Affiliated bacteriab
EUB338 (mean ± SD)ALF1bBET42aGAM42aCF319aPLA30SRB385HGC69aARC94DNMA657
Mud core
 0.544 ± 873.0 ± 63.54.13.618.13.54.43.21.10.341.5
 1.045 ± 465.0 ± 34.74.04.716.35.74.33.61.00.244.3
 1.546 ± 454.0 ± 54.54.33.513.05.45.12.31.10.439.2
 2.045 ± 343.5 ± 114.33.73.412.14.56.53.11.30.638.9
 2.543 ± 254.5 ± 34.44.03.413.64.45.32.60.90.538.6
 3.045 ± 641.8 ± 73.93.83.312.04.04.32.40.10.533.8
 3.533 ± 541.3 ± 43.13.03.411.83.36.12.50.010.333.2
 4.024 ± 540.0 ± 82.52.83.58.12.25.61.70.020.226.4
 4.527 ± 537.0 ± 92.72.03.46.41.33.51.00.010.420.3
 5.018 ± 428.6 ± 72.32.03.36.21.32.00.10.010.317.2
Beach core
 0.56.1 ± 940.0 ± 61.51.00.87.52.82.90.90.90.218.3
 1.07.8 ± 745.0 ± 32.01.21.37.73.03.51.40.90.321.1
 1.525 ± 354.0 ± 23.53.83.08.53.73.81.61.50.429.4
 2.032 ± 450.0 ± 73.33.53.28.74.14.21.31.60.329.9
 2.522 ± 543.5 ± 53.43.63.18.33.25.21.21.30.229.3
 3.023 ± 641.2 ± 22.93.32.86.23.44.91.01.00.425.5
 3.520 ± 740.0 ± 112.53.02.75.73.13.60.40.80.221.8
 4.012 ± 435.0 ± 92.32.82.65.02.82.30.80.30.318.9
  • a Oligonucleotide probes (formamide concentration used for experiments): domain Eukarya, EUK516 (20%); domain Archaea, ARCH915 (20%); domainBacteria, EUB338 (35%); α subclass ofProteobacteria, ALF1b (20%); β subclass ofProteobacteria, BET42a (35%); γ subclass ofProteobacteria, GAM42a (35%); sulfate reducers of the δ subclass of Proteobacteria, SRB385 (20%);Cytophaga-Flavobacterium cluster, CF319a (35%);Planctomycetes, PLA30 (20%); gram-positive bacteria with high G+C content, HGC69a (20%); Arcobacter spp., ARC94 (20%) (40, 51); Desulfonema magnum, D. limicola, and D. ishimotoei, DNMA657 (35%) (12). Probes BET42a, GAM42a, and PLA30 were used with competitor (22, 27).

  • b Results from the addition of counts with the following specific probes: ALF1b, BET42a, GAM42a, SRB385, CF319a, PLA30, HGC69a, and ARC94.