Table 3.

Results of experimental specificity testing of pure cultures with the Ps-PCR primers

Culture descriptionaHaeIII RFLP pattern
Ps-PCRbUniversal PCRc
Culture collections
P. putida type B ATCC 17527*APsm.A
P. putida ATCC 11172APsm.A
P. aeruginosa ATCC 10145APsm.A
P. syringae pv. syringae Biolog 7350*APsm.A
P. syringae pv. syringae EPA 22A-93APsm.A
P. syringae pv. tomato EPA PT-23APsm.A
P. syringae pv. phaseolica EPA JA-019APsm.A
P. cichorii ATCC 10857*APsm.A
P. taetrolens Biolog 8570*APsm.A
P. synxantha ATCC 9890*APsm.A
P. fragi ATCC 4973*APsm.A
P. resinovorans ATCC 14235*APsm.A
P. mendocina ATCC 25411*APsm.A
P. agarici ATCC 25941*BPsm.B
P. fulva ATCC 31418*APsm.A
P. stutzeri ATCC 17588*APsm.A
P. alcaligenes B ATCC 14909*APsm.A
P. aureofaciens EPA 604APsm.A
Burkholderia pickettii ATCC 27511BuhI.A
Burkholderia andropogonis ATCC 23061*BuhII.A
Burkholderia cepacia ATCC 25416BuhII.A
Comamonas testosteroni ATCC 11996Com.A
Acidovorax facilis ATCC 11228Acv.A
Hydrogenophaga flava ATCC 33667Hyp.A
Brevundimonas vesicularis ATCC 11426Brm.A
Stenotrophomonas maltophila ATCC 13637Stm.A
P. floridana ATCC 25616*“Psm”.A
Pseudomonas species-like group II Biolog 1574*“Psm”.A
P. boreopolis ATCC 3242*“Psm”.B
Biolog-identified environmental isolates
P. putida A1 isolates E8P3D2, E12P3C1, F2P4B3, and 77ysAPsm.A
P. putida B1 isolates F2C1B1, BMD B2, and BMD B7APsm.A
P. fluorescens type A isolate E2P5B1APsm.A
P. fluorescens type B isolates 75csr, 137ymr, 182ysr, and 228csrAPsm.A
P. fluorescenstype C isolate F4C3D3APsm.A
P. fluorescens type G isolates E2P5A3, F2C1B3, 27cm, 129,ymr, 7A, and 2AAPsm.A
P. marginalis isolates A5P4C3 and 18BCPsm.A
P. corrugata isolates E8C1D1 and 104ysrAPsm.A
P. viridilividaisolates E2P4B3 and E2P4A1APsm.A
P. aurantiaca isolates RES A5 and FB49HAPsm.A
P. viridiflava isolate RES A7APsm.A
P. fragi isolate 7CAPsm.A
P. fuscovaginae isolate 7BAPsm.A
Pseudomonas sp. isolate RES A4APsm.A
Pseudomonas sp. isolate BMD B36APsm.A
Acinetobacter sp. isolate 78 csAcb.A
Acinetobacter calcoacitices isolate FA18CAcb.A
Alcaligenes xylosoxidans isolate FA30GAlc.A
  • a Fifty Pseudomonas and 14 non-Pseudomonas cultures used to test the specificity of the Ps-PCR protocol. ∗, culture DNA sample kindly provided by Biolog Inc.

  • b The HaeIII RFLP patterns of Ps-PCR products. The three detected patterns (as shown in Fig. 1b2) were labelled A, B, and C. −, cultures that did not yield an amplification product by the Ps-PCR protocol with 30 cycles at an annealing temperature of 68°C.

  • c The HaeIII RFLP patterns of PCR products obtained with the universal SSU rRNA gene primer set. Patterns (as shown in Fig. 1a2) were labelled with a genus abbreviation followed by a capital letter. Psm, Pseudomonas; BuhI,Burkholderia type I; BuhII, Burkholderia type II; Com, Comamonas; Hyp, Hydrogenophaga; Brm,Brevundimonas; Stm, Stenotrophomonas; Acb,Acinetobacter; Alc, Alcaligenes; quotation marks, non-Pseudomonas (sensu stricto) cultures that are still named Pseudomonas.