Table 2.

PCR temperature cycling conditions on an MJR PTC-100 operated with block control

MethodSample typeTemp (°C)a under the following conditions and incubation timesb:
DenaturationAnnealingExtension
11 s15 s8 s1 min10 s1 min
Ps-PCRc Cultures949266687472
Ps-PCRc Soil DNA949263657472
uni-PCRd Cultures949260627472
  • a Target block temperature for which the temperature cycler was programmed.

  • b Time period for which the block of the temperature cycler was maintained at the given target temperature.

  • c PCR performed with primers Ps-for and Ps-rev.

  • d PCR performed with the universal SSU primers uni-for and uni-rev.