Table 2.

Activity of the enzyme system during partial purification

Purification stepSp acta (fold purification)
PNP4-Nitrocatechol
Cell extraction1.3 1.3 
DEAE-Sepharose chromatography7.5 (6)7.1 (5)
Q-Sepharose chromatography19.4 (15)13.5 (10)
Sephacryl S-300 chromatographyb 40.0 (32)38.0 (28)
  • a Nanomoles of nitrite per milligram of protein per minute.

  • b Combination of 104- and 114-ml elution volumes.