Table 2.

Comparison of virus numbers obtained with different dilutions of a Phaeocystis virus suspension fixed with 0.5% glutaraldehyde and immediately frozen in liquid nitrogen and analyzed by FCM after staining with SYBR-I or by EFM after staining with DAPI or SYBR-Ia

DilutionViral counts (105/ml) by:
FCM with SYBR-IEFM
DAPI, 0.2-μm black filterDAPI, 0.02-μm white filterSYBR-I, 0.02-μm white filter
1460.0430.0430.0
1018.9836.037.037.0
507.01
1006.404.403.704.90
2502.71
5001.411.011.101.10
7500.97
1,0000.740.480.48
2,0000.39
5,0000.16
10,0000.12
25,0000.03
50,0000.02
100,0000.02
  • a For EFM, the samples were filtered onto either black 0.2-μm-pore-size or white 0.02-μm-pore-size filters.