TABLE 1

Yeast aldehyde reductases and their kinetic constants toward preferred substrates

Enzyme (reference)Cofactor preferenceKm (μM)Aliphatic aldehyde substrateaAryl-aldehyde substratea
SubstrateKm (μM)kcat (min−1)SubstrateKm (μM)kcat (min−1)
PcAad1 (10)NADPH39HexanalNA2473,4-Dimethoxybenzaldehyde12530
HeptaldehydeNA138Benzaldehyde1,700430
Cinnamaldehyde3,400670
Aad4bNADPHNAHexanalNA1723,4-DimethoxybenzaldehydeNA142
HeptaldehydeNA644-NitrobenzaldehydeNRNR
Aad14bNADPHNAHexanalNA504-NitrobenzaldehydeNA22
HeptaldehydeNA46CinnamaldehydeNRNR
Aad10-35CbNADPHNAHexanalNRNR4-NitrobenzaldehydeNRNR
HeptaldehydeNRNRCinnamaldehydeNRNR
Aad6518GbNADPHNAHexanalNRNR4-NitrobenzaldehydeNRNR
HeptaldehydeNRNRCinnamaldehydeNRNR
Adh6 (25)NADPH29Hexanal15221,270Cinnamaldehyde17218,400
Pentanal6022,700Veratraldehyde7315,800
Adh7 (26)NADPHNAPentanal4911,915Cinnamaldehyde437,913
3-Methylbutanal489,581Veratraldehyde586,000
Gre3 (27)NADPH13Hexanal3,1001094-Nitrobenzaldehyde120142
Gcy1 (28)NADPH12Butyraldehyde5,400814-Nitrobenzaldehyde13071
Benzaldehyde5,20058
Ypr1 (29)NADPH8.7Hexanal3903544-Nitrobenzaldehyde1,0701,776
2-Methylbutyraldehyde1,0905249,10-Phenanthrequinone2,600272
Yjr096w (28)NADPH370Butyraldehyde1,8000.54-Nitrobenzaldehyde50088
Benzaldehyde4,7004
Ydl124w (28)NADPH23Butyraldehyde210,000144-Nitrobenzaldehyde303.3
Benzaldehyde2404
  • a kcat as reported or normalized based on reported Vmax. NA, data not available; NR, not reactive.

  • b Results of this study.