Table 1.

Probe sequences, target sites, and formamide concentrations in the hybridization buffer required for specific in situ hybridization

ProbeSequence (5′–3′)Target sitea (16S rRNA positions)% Formamide
Nso190CGATCCCCTGCTTTTCTCC190–20855
Nso1225CGCCATTGTATTACGTGTGA1225–124435
Nsv443CCGTGACCGTTTCGTTCCG444–46230
Nsm156TATTAGCACATCTTTCGAT156–1745
NEUCCCCTCTGCTGCACTCTA653–67040
CTETTCCATCCCCCTCTGCCG659–676b
S-*-Nse-1472-a-A-18ACCCCAGTCATGACCCCC1472–148950
NmVTCCTCAGAGACTACGCGG174–19135
S-*-Ntspa-1026-a-A-18AGCACGCTGGTATTGCTA1026–104320
NIT3CCTGTGCTCCATGCTCCG1035–104840
CNIT3CCTGTGCTCCAGGCTCCG1035–1048c
  • a E. coli numbering (10).

  • b Used as unlabeled competitor together with probe NEU.

  • c Used as unlabeled competitor together with probe NIT3.