Table 4.

Enzymatic activities of culture broth versus HPLC-purified P. luminescens insecticidal toxin

ActivityActivity ofa:
Culture brothHPLC-purified toxin
Protease0.680
Lipase
 Ca precipitate+
 Colorimetric5.50.2
Hemolysin+
Phospholipase C+
Nuclease+
  • a +, activity present; −, no activity present. Units of activity for protease: change in optical density of an azocasein solution, 2 h; units of activity for lipase (colorimetric): micromoles of p-nitrophenol liberated fromp-nitrophenyl palmitate in 30 min.