Table 3.

Toxicities of injected or orally administered HPLC-purified toxin from P. luminescens W-14 for fourth-instar M. sexta larvae

Samplea% MortalitybInitial wt (g) ± SEFinal wt (g) ± SEFold weight gainb
Fedc
 Control buffer00.23 ± 0.026.15 ± 0.4826.7
 0.2 μg00.26 ± 0.022.74 ± 0.5011.0
 2.0 μg00.25 ± 0.030.68 ± 0.142.7
 20 μg750.24 ± 0.030.20 ± 0.010.8
Injectedd
 Control buffer00.56 ± 0.028.02 ± 0.3014.4
 0.05 μg580.52 ± 0.031.02 ± 0.071.9
 0.5 μg100NANA NAe
 5 μg100f NANANA
  • a n = 12 larvae for each sample group.

  • b At 6 days posttreatment.

  • c Toxin was applied to 1 cm2 of diet in 50 μl of KPO4 buffer, pH 6.9.

  • d Toxin was injected in 5 μl of KPO4 buffer, pH 6.9.

  • e NA, not applicable (all larvae died).

  • f All larvae were dead by 24 h postinjection.