Table 3.

Phylogenetic affiliation of clones obtained from attached and free-living bacteria at DCM

Best matchaSimilarity (%)bNo. of clonescNo. of nucleotides compared (minimum–maximum)Phylogenetic affiliation
ATT (no. of clones = 18)
A. macleodii IAM 12920T92.5–98.915564–828γProteobacteria
A. macleodii IAM 12920T94.41
Unidentified αProteobacteria; clone SAR 407; cluster SAR1192.51796α Proteobacteria
Unidentified α Proteobacteria; clone SAR 407; cluster SAR11921684
Free (no. of clones = 24)
Uncultured proteobacterium OCS5; cluster SAR8691.1–99  3533–567γProteobacteria
Uncultured proteobacterium OCS5; cluster SAR8693.21634
Uncultured proteobacterium OCS5; cluster SAR8692.21773
Uncultured proteobacterium OCS44; cluster SAR86  93–96.62641–767
 Proteobacterial SCB1192.71508
A. macleodii IAM 12900T961756
A. macleodii IAM 12900T97.11650
P. agarici921724
Aeromonas sp.96.51734
Unidentified α Proteobacteria; clone SAR 407; cluster SAR1185.7–98  2530–617αProteobacteria
Unidentified αProteobacteria; clone SAR 220; cluster SAR1190.81591
Uncultured proteobacterium; OCS180; cluster SAR11951670
Paracoccus solventivorans88.51609
Sulfitobacter pontiacus ChLG-1094.81582
Microcystis elabens NIES4291.81723Cyanobacteria
Unknown marine bacterioplankton; clone SAR7961565
Psychroserpens burtonensisACAM18886.21764Cytophagales
Gelidibacter algens C8ST582.41579
Unidentified proteobacterium ARC33931685β Proteobacteria
Unidentified firmicute OM196.61682High G+C gram positive
  • a Name in boldface given by authors depositing the sequence and corresponding to uncultured bacteria. ATT, attached; Free, free-living.

  • b The range of similarities indicates the highest and lowest values of similarity to the best match when more than one clone with over 97% identity was found.

  • c Clones with more than 97% nucleotide identity were considered identical.