Table 2.

Oligonucleotide probe sequences and hybridization temperatures used for the probe analysis of clone libraries obtained from particle-associated and planktonic samples from the northwestern Mediterranean Seaa

ProbeSequence (5′-3′)Target groupTemp (°C)
β-AO233AGCTAATCAGRCATCGGAll β-subgroup ammonia oxidizers44
Nsp436TTTCGTTCCGGCTGAAAGAll Nitrosospiraspp.45
Nmo254GTAGGCCSTTACCCYACCAllNitrosomonas spp.43
  • a The probes were designed and optimized by Stephen et al. (40).