Table 3.

Percentages of clones in clone libraries derived from particle-associated and planktonic material from the northwestern Mediterranean Sea that hybridized with probes β-AO233, Nsp436, and Nmo254

Clone libraryNo. of clonesClones that hybridized witha:
β-AO233Nsp436Nmo254
No.%No.%No.%
100 FREE90000000
100 AGG9089008100
400 FREE892326229614
400 AGG9015170015100
700 FREE9167117583
700 AGG877283346996
  • a The results were corrected for clones which gave unexpected probe profiles and which after sequence analysis proved to be closely related to non-ammonia-oxidizing species. The values for Nsp436 and Nmo254 were calculated as percentages of the number of β-AO233-positive clones.