TABLE 5

Kinetic characterization and thermostability determination of best MhIHL variants

ProteinKm (mM)kcat (s−1)kcat/Km (s−1 · mM−1)T5015 (°C)E value
Wild type12 ± 2200 ± 2015.77410
Glu95Gln-Val54Ser mutant24 ± 2143 ± 105.967178
Glu95Gln-Val54Leu mutant28.1 ± 1.2101 ± 73.665199
Glu95Val-Val54Ser mutant18 ± 2120 ± 136.562187
Glu95Val-Val54Leu mutant26.3 ± 1.1168 ± 86.461>200
Arg162Thr-Val54Ser mutant18 ± 2130 ± 107.266>200
Arg162Thr-Val54Leu mutant20.2 ± 0.9159 ± 107.969>200
Glu95Lys-Val54Ser mutant20 ± 2164 ± 108.270>200
Glu95Lys-Val54Leu mutant20.2 ± 1.9123 ± 86.168>200