TABLE 4

T5015 values of MhIHL and its mutants

ProteinT5015 (°C)a
Wild type41
Glu95Phe mutant60
Glu95Gln mutant70
Glu95Val mutant58
Arg162Thr mutant57
Glu95Lys mutant72
Gly42Thr-Val56Met-Gly57Val mutant48
  • a T5015, the temperature at which 50% of the enzyme activity is lost following heat treatment for 15 min. The enzyme was incubated at different temperatures (30 to 75°C) for 15 min, followed by measuring of the residual activity.