Table 1.

Oligonucleotide primers used for PCR amplification of human adenovirus, enterovirus, and HAV

Virus type (region)aPositionAmplification reactionPrimersProduct size (bp)Sequence
Ad2 (hexon)
Ad40 (hexon)18858–18883b FirsthexAA18853015′-GCCGCAGTGGTCTTACATGCACATC-3′
Ad41 (hexon)19136–19158b FirsthexAA19135′-CAGCACGCCGCGGATGTCAAAGT-3′
Ad2 (hexon)18937–18960b NestednehexAA18931435′-GCCACCGAGACGTACTTCAGCCTG-3′
Ad2 (hexon)19051–19079b NestednehexAA19055′-TTGTACGAGTACGCGGTATCCTCGCGGTC-3′
Polio 1 (5′NTR)
CV B4 (5′NTR)64–83c FirstEnt 1d 5405′-CGGTACCTTTGTACGCCTGT-3′
Polio 1 (5′NTR)578–597c FirstEnt 25′-ATTGTCACCATAAGCAGCCA-3′
Polio 1 (5′NTR)430–450c NestedneEnt 11235′-TCCGGCCCCTGAATGCGGCTA-3′
CV B4 (5′NTR)547–567c NestedneEnt 25′-GAAACACGGACACCCAAAGTA-3′
HAV (5′NTR)332–352FirstHAV13685′-TTGGAACGTCACCTTGCAGTG-3′
HAV (5′NTR)680–700FirstHAV25′-CTGAGTACCTCAGAGGCAAAC-3′
HAV (5′NTR)371–391NestedneHAV12905′-ATCTCTTTGATCTTCCACAAG-3′
HAV (5′NTR)641–661NestedneHAV25′-GAACAGTCCAGCTGTCAATGG-3′
  • a Ad, adenovirus; CV, coxsackievirus.

  • b Sequence position refers to the Ad2 hexon region.

  • c Sequence position refers to the coxsackievirus B4 5′NTR.

  • d Modified from Gow et al. (9).