Table 1.

Organisms used in dot blot specificity studies and results of FISH specificity tests

StrainaOriginDot blotb and FISHc results with indicated probe
Bfra602Bdis656Erec482Chis150Clit135Strc493
Bacteroides fragilisDSM 2151+, P−, N−, N−, N−, N−, N
Bacteroides vulgatusDSM 1447+, P−, N−, N−, N−, N−, N
Bacteroides eggerthiiDSM 20697+, P−, N−, N−, N−, N−, N
Bacteroides sp. (B. fragilis)MMB+, P−, N
Bacteroides sp. (B. thetaiotaomicron)MMB+, P−, N
Bacteroides sp. (B. vulgatus)MMB+, P−, N
Bacteroides sp. (B. eggerthii)MMB+
Bacteroides sp. (B. uniformis)MMB+
Cytophaga xylanolyticaDSM 6779+/−, P−, N−, N−, N−, N−, N
Bacteroides distasonisDSM 20701−, N+, P−, N−, N−, N−, N
Bacteroides sp. (B. distasonis)MMB−, N+, P
Clostridium oroticumDSM 1287−, N−, N+, P−, N−, N−, N
Clostridium sp. (C. nexile)MMB+, P
Ruminococcus hanseniiDSM 20583+, P
Ruminococcus productusDSM 2950+, P
Eubacterium ventriosumDSM 3988+
Clostridium sp. (C. clostridiiforme)MMB+
Clostridium histolyticumDSM 2158−, N−, N−, N+, P−, N−, N
Clostridium sp. (C. beijerinckii)MMB+
Clostridium sp. (C. butyricum)MMB+, P−, N
Clostridium sp. (C. perfringens)MMB+, P−, N
Clostridium putrificumDSM 1734+, P
Clostridium sp. (C. cadaveris)MMB+, P/N
Clostridium difficileATCC 9688−, N−, N−, N−, N+, P/N−, N
Eubacterium tenueDSM 20695−, N+, P−, N
Clostridium bifermentansNIZO B529−, N+, P−, N
Clostridium sp. (C. sordellii)MMB+
Peptostreptococcus (P. anaerobius)MMB−, N−, N
Fusobacterium nucleatumd DSM 20482−, N−, N−, N−, N−, N−, N
Eubacterium plautiiDSM 4000−, N−, N−, N−, N−, N−, N
Eubacterium sp. (E. cylindroides)MMB
Streptococcus sanguisDSM 20567−, N−, N−, N−, N−, N+, P
Streptococcus oralisDSM 20627−, N−, N−, N−, N−, N+, P
Streptococcus intermediusDSM 20573+, P
Lactococcus lactis subsp. cremorisDSM 20069−, N−, N−, N−, N−, N+, P
Streptococcus sp. (S. mitis)MMB−, N−, N−, N−, N−, N+, P
Leuconostoc lactisNIZO B630+, P
Streptococcus sp. (S. bovis)MMB+, P
Streptococcus sp. (S. equi subsp.equi)MMB+
Streptococcus mutansDSM 20523+
Streptococcus sp. (S. sanguis)MMB+
Streptococcus sp. (S. salivarius)MMB+
Streptococcus sp. (S. equinus)MMB+
Streptococcus sp. (S. pyogenes)MMB+
Enterococcus faecalisDSM 20478−, N−, N−, N−, N−, N−, N
Enterococcus gallinarumDSM 20628
Lactobacillus acidophilusNIZO B228−, N
Weissella kandleriDSM 20593N
Lactobacillus fermentumDSM 20052
Vagococcus fluvialisDSM 5731−, N−, N−, N−, N−, N−, N
Bifidobacterium infantisATCC 15697−, N−, N−, N−, N−, N−, N
Bifidobacterium dentiumATCC 27678
Bifidobacterium sp. (B. longum)MMB−, N−, N
Bifidobacterium adolescentisNIZO B659
Bifidobacterium pseudolongumNIZO B669
Escherichia coliDSM 25922−, N−, N−, N−, N−, N−, N
Carnobacterium divergensDSM 20623−, N−, N−, N−, N−, N−, N
Lactobacillus maltaromicusDSM 20342
Salmonella sp. (S. typhi)MMB
Enterobacter sp. (E. aerogenes)MMB
Serratia sp. (S. marcescens)MMB
Proteus sp. (P. vulgaris)MMB
Klebsiella sp. (K. pneumoniae)MMB
  • a All strains with species name in parentheses are clinical and human fecal isolates identified as indicated.

  • b For dot blot analysis, the symbols +, −, and +/− indicate that the strains gave positive, negative, and ambiguous signals, respectively.

  • c The letters indicate that these strains were used in FISH tests and hybridized positively (P), negatively (N), or ambiguously (P/N) with the corresponding probes.

  • d Fusobacterium nucleatum subsp.polymorphum.