Table 2.

Enumeration of L. plantarum GFP+bacteria by flow cytometry or plate counts

Type of countBacterial count of L. plantarum GFP+ bacteria at dilutionc:
10−310−410−510−6
Plate count (CFU/ml)1.1 × 106 1.3 × 105 1.6 × 104 1.0 × 103
FACS count (no. of events/ml)a 1.6 × 106 2.0 × 105 1.6 × 104 1.2 × 103
FACS count in a mixed population (no. of events/ml)b 1.9 × 106 1.8 × 105 1.8 × 104 1.7 × 103
  • a One hundred microliters of L. plantarum GFP+ bacteria was resuspended in 900 μl of PBS, and serial dilutions were analyzed by fluorescence-activated cell sorting (FACS).

  • b One hundred microliters of L. plantarum GFP+ and 100 μl of L. plantarumGFP bacteria were added to 800 μl of PBS. Serial dilutions of this mixed population were analyzed by fluorescence-activated cell sorting.

  • c The numbers in the table represent the mean values of three independent experiments.