Table 1.

Strains and plasmids

Strain or plasmidDescriptionaReference or source
Strains
E. coli DH10BF-mcrAΔ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara,leu)7697 galU galK1 rpsL nupG Life Technologies, Inc. (Gaithersburg, Md.)
E. coli33456Wild type 13
Pseudomonassp. strain GJ1 9
Plasmids
 pGFPuvpUC-based vector carrying gfpuv, Apr Clontech
 pGFPmut3pKEN containinggfpmut3, Apr 4
 pCSAK50pBAD-based vector containing gfpuv, Apr A. Khlebnikov and J. D. Keasling, unpublished data
 pSMC21b pUCP-based plasmid containing gfpmut2, Apr, Cbr, Kmr 2
  • a Apr, ampicillin resistance; Cbr, carbenicillin resistance; Kmr, kanamycin resistance.

  • b pSMC21 is identical to pSMC2 but has an additional Kmr gene.