Table 1.

Oligonucleotide primers used for PCR amplification and sequencing of human Ad and BKVa

Virus type (region)Position (nt)Amplification reactionPrimerProduct size (bp)Annealing temp (°C)Sequence
Ad2 (hexon)
Ad40 (hexon)18858–18883First-leftHL301555′-GCCGCAGTGGTCTTACATGCACATC-3′
Ad41 (hexon)19136–19158First-rightHR5′-CAGCACGCCGCGGATGTCCAAAGT-3′
Ad2 (hexon)18937–18960Nested-leftNHL143555′-GCCACCGAGACGTACTTCAGCCTG-3′
Ad2 (hexon)19051–19079Nested-rightNHR5′-TGTACGAGTACGCGGTATCCTCGCGGTC-3′
BKV, JCV (VP2/VP3)1352–1367First-leftBJ1765465′-TATTGCMCCAGGAGGT-3′
BKV, JCV (VP1)2132–2148First-rightBJ25′-AACATTTTCYCCTCCTG-3′
BKV (VP1)1762–178Nested-leftBK4290505′-AGTAGATTTCCACAGGTTAG-3′
BKV (VP1)1486–1506Nested-rightBK65′-CCAGGGGCAGCTCCCAAAAAG-3′
BKV (VP1)2132–2148Seminested-leftBK2388465′-AACATTTTCCCCTCCTG-3′
BKV (VP1)1758–1776Seminested-rightBK55′-GGACCTAACCTGTGGAAAT-3′
  • a M = A + C; Y = C + T. For Ad, the sequence positions are referred to the Ad2 hexon region; for BKV and JCV, the sequence positions are referred to the BKV-Dunlop sequence.