Table 2.

Results of PCR amplifications with the different sets of primers for nirKa

Species or strainbSource or referencebDenitrificationcnirKc PCR productsd determined with primers:
nirK1F-nirK3RnirK1F-nirK4RnirK1F-nirK5RnirK2F-nirK3RnirK2F-nirK4RnirK2F-nirK5R
Alcaligenes xylosoxidans NCIMB 11015NCIMB+++++
Hyphomicrobium zavarzinii IFAM ZV-622T (ATCC 27496)ATCC++++
Ochrobactrum anthropi LMG 2136LMG++++++++
Rhizobium meliloti Rm1021C. Elmerich, Institut Pasteur, Paris, France++++++
Rhizobium meliloti 20115C. Elmerich, Institut Pasteur, Paris, France++++++
Rhodobacter sphaeroides f. sp. denitrificans Satoh et al. (20)+++++e +
Alcaligenes sp. strain (DSM 30128)DSM+NDg ++++e +
Alcaligenes xylosoxidans subsp.denitrificans DSM 30026DSM+ND+++
Blastobacter denitrificans IFAM 1005 (DSM 1113)DSM+ND+++
Enterobacter agglomerans 339W. Klingmüller, Universität Bayreuth, Bayreuth, Germanyf f f f
Enterobacter cloacae NCIMB 11463NCIMBf
Escherichia coli K-12 (DSM 498)DSMf f f f
Klebsiella pneumoniae subsp. pneumoniae DSM 681DSMf f
  • a Touchdown reaction from 45 to 40°C used.

  • b ATCC, American Type Culture Collection; DSM, Deutsche Sammlung von Mikroorganismen; IFAM, Institut für Allgemeine Mikrobiologie, Universität Kiel, Kiel, Germany. LMG, Laboratorium voor Mikrobiologie, Universiteit Gent, Ghent, Belgium; NCIMB, National Collections of Industrial and Marine Bacteria, Aberdeen, United Kingdom.

  • c Data from literature.

  • d +, PCR product of the expected size; −, no PCR product.

  • e Expected PCR products and weaker products of unexpected and nonspecific size.

  • f No expected PCR products, only products of unexpected and nonspecific size.

  • g ND, not determined.