Table 1.

Primer sequences and positions used to amplify fragments from nirK and nirS nitrite reductase

PrimeraPositionbPrimer sequence (5′-3′)
nirK1F526–542GG(A/C)ATGGT(G/T)CC(C/G)TGGCA
nirK2F565–581GC(C/G)(C/A)T(C/G)ATGGT(C/G)CTGCC
nirK3R898–918GAACTTGCCGGT(A/C/G)G(C/T)CCAGAC
nirK4R942–959GG(A/G)AT(A/G)A(A/G)CCAGGTTTCC
nirK5R1023–1040GCCTCGATCAG(A/G)TT(A/G)TGG
nirS1F763–780CCTA(C/T)TGGCCGCC(A/G)CA(A/G)T
nirS2F855–874TACCACCC(C/G)GA(A/G)CCGCGCGT
nirS3F1002–1019TTCCT(C/G/T)CA(C/T)GACGGCGGC
nirS4F1317–1336TTC(A/G)TCAAGAC(C/G)CA(C/T)CCGAA
nirS3R1002–1019GCCGCCGTC(A/G)TG(A/C/G)AGGAA
nirS5R1494–1514CTTGTTG(A/T)ACTCG(C/G)(C/G)CTGCAC
nirS6R1638–1653CGTTGAACTT(A/G)CCGGT
  • a The primers are indicated by nirK for the nirK gene and nirS for the nirS gene; forward and reverse primers are indicated by the last letters F and R, respectively.

  • b Positions in the nirK gene ofAlcaligenes faecalis S-6 EMBL D13155 and in thenirS gene of Pseudomonas stutzeri ZoBell EMBLX56813.