Table 1.

APase specific activities under different conditions of planktonic and biofilm P. aeruginosa organisms

BacteriaConditionsaEnzyme sp act (ΔA410 mg of protein−1min−1)bCell densityc
PlanktonicHP for 8 h 0   1.52 × 109
LP for 8 h59.19 2.6 × 108
BiofilmLP + air for 8 h3.95 ± 0.564.26 ± 0.96 × 109
LP + pure N2 for 8 h0.13 ± 0.0794.43 ± 1.43 × 109
LP + pure O2 for 8 h7.49 ± 0.834.72 ± 0.53 × 109
  • a HP, growth in high-phosphate medium; LP, growth in low-phosphate medium.

  • b ΔA 410, change inA 410.

  • c Cell densities are expressed as CFU per milliliter of planktonic bacteria and CFU per square centimeter of biofilm.