Table 1.

AATFase and Iah1p activities in cells grown in YPD medium

Strain/plasmidActivity (U/mg of protein)aNo. of copiesb
AATFaseIah1pATF1IAH1
YPHT/YEp241.2 ± 0.40.42 ± 0.0911
YPT1/YEp241.2 ± 0.30.013 ± 0.0310
YPHT/YEp24-ATF118 ± 6.50.44 ± 0.09161
YPT1/YEp24-ATF120 ± 6.50.012 ± 0.02170
YPHT/YEp24-IAH11.1 ± 0.33.3 ± 0.3319
  • a One unit of AATFase activity is defined as the amount of enzyme forming 1 ppm of isoamyl acetate per h at 25°C (4). One unit of Iah1p activity is defined as the amount of enzyme forming 1 ppm of acetate per h at 25°C (3). Values are means ± standard deviations from four different tests.

  • b Copy number is the sum of the number of plasmid copies and endogeneous copies.