Table 3.

Results of PCR amplifications with the different sets of primers for nirSa

StrainbSource or referencebDenitrificationcnirSc PCR productsd determined with primers:
nirS1F-nirS3RnirS1F-nirS5RnirS1F-nirS6RnirS2F-nirS3RnirS2F-nirS5RnirS2F-nirS6RnirS3F-nirS5RnirS3F-nirS6RnirS4F-nirS5RnirS4F-nirS6R
Alcaligenes eutropus H16 (DSM 428)DSM+++++++
Alcaligenes faecalis A15H. Bothe, Universität Köln, Cologne, Germany+++++++++
Azospirillum brasilense Sp7 (DSM 1690)DSM++++++
Paracoccus denitrificans ATCC 19367ATCC++++++++++
Pseudomonas aeruginosa DSM 6195DSM+++e ++
Pseudomonas stutzeri ATCC 14405ATCC++++++++++++
Roseobacter denitrificans ATCC 33942T ATCC++++++++e ++
Alcaligenes eutrophus DSM 530DSM+NDg +++++
Denitrifying isolate IFAM 3698IFAM+ND++++e ++
Escherichia coli K-12 (DSM 498)DSMf f f f f f
Enterobacter agglomerans 339W. Klingmüller, Universität Bayreuth, Bayreuth, Germanyf f f
Enterobacter cloacae NCIMB 11463NCIMBf f
Klebsiella pneumoniae subsp. pneumoniae DSM 681DSM
  • a Touchdown reaction from 45 to 40°C used.

  • b See Table 2, footnote b.

  • c Data from literature.

  • d +, PCR product of the expected size; −, no PCR product.

  • e Expected PCR products and weaker products of unexpected and nonspecific size.

  • f No expected PCR products, only products of unexpected and nonspecific size.

  • g ND, not determined.