TABLE 2

Recovery of enzyme activity from pseudogenized ScAad10p and ScAad6/16p following ancestral state reconstructiona

EnzymePcAad1pScAad10-35CpScAad6518Gp
VmaxKmVmaxKmVmaxKm
4-Methoxybenzaldehyde6.63 ± 0.1287.3 ± 1160.08 ± 0.01157 ± 39.80.55 ± 0.02217 ± 26.1
Hydroxymethylfurfural2.40 ± 0.27171 ± 36.70.37 ± 0.07608 ± 246NR
Phenylacetaldehyde9.00 ± 0.27527 ± 72.50.30 ± 0.0817.6 ± 2.200.49 ± 0.02296 ± 29.6
  • a Vmax (in micromoles per minute per milligram) and Km (micromolar) values are shown as the mean ± standard error (SE) of the results from triplicate experiments. NR, not reactive.