TABLE 1

Origin, BOX-PCR, ITS (16S-23S rRNA) characterization, nodulation status and colony morphology of symbiotic bacteria isolated from the root nodules of cowpea from Mozambiquea

Experimental siteIsolateCowpea varietyBOX-PCR clusterITS band size (bp)ITS typeITS-RFLP type (HaeII, HindIII, HinfI)Colony morphologyNodulation assay
Days to emergenceColorSize (mm)
MuriazeTUTVU1IT-18VIII1,000IXAA-5–7Milky1.2+
TUTVU2IT-18VIII1,107XBBA5–7White0.9+
TUTVU3IT-1263VIII1,000IXCCB5–7Milky1.0+
TUTVU4IT-18VII500IIDDC5–7Milky1.0+
TUTVU5IT-18VII1,000IXEED5–7Milky1.0+
TUTVU6IT-1263VII1,000IXEED5–7Milky1.1+
TUTVU7IT-18IV1,000IXFCD5–7Milky1.0+
TUTVU8IT-18IV500IIDDE7–10White1.0+
TUTVU9IT-1263V1,000IXAAD5–7White0.8+
TUTVU10IT-1263IV1,000IXCCD5–7Milky0.8+
TUTVU11IT-18XV1,000IXCCD5–7Translucent0.9+
TUTVU12IT-1263XV1,000IXAAD5–7Translucent0.9+
TUTVU13IT-1263VI1,000IXAAD5–7White0.9+
TUTVU14IT-18XI1,000IXCCD7–10Milky0.7+
TUTVU15IT-18XV1,000IXGCD7–10Milky0.8+
TUTVU16IT-1263XV1,000IXGCD5–7Milky0.8+
TUTVU17IT-1263X1,000, 500XVIHED5–7Milky0.6+
TUTVU18IT-18IX1,000IXEAD7–10Milky0.7+
TUTVU19IT-1263IX1,000IXEAD5–7Milky1.2+
TUTVU20IT-1263IX1,000IXEAD5–7Milky1.2+
TUTVU21IT-1263II1,000IXGCD5–7Translucent1.1+
TUTVU22IT-1263II1,000IXCCD5–7Translucent1.1+
TUTVU23IT-1263II1,000IXCCD5–7Translucent1.1+
TUTVU24IT-1263II1,000IXCCD5–7Translucent1.1+
TUTVU25IT-18XVI1,000IXCCD5–7Milky1.1+
TUTVU26IT-18XVI1,000IXCCD5–7Milky1.1+
TUTVU27IT-18XVI1,000IXCCD5–7Milky1.0+
TUTVU28IT-18XVI1,000IXCCD5–7White1.0+
TUTVU-1IT-18VIII7–10Milky0.7
TUTVU-2IT-18XVI7–10Milky1.8
TUTVU-3IT-18XV5–7Milky1.8
TUTVU-4IT-1263SA5–7Milky1.7
TUTVU-5IT-1263SA5–7Milky1.7
TUTVU-6IT-1263SA5–7Milky1.7
TUTVU-7IT-1263XVII5–7Milky1.6
TUTVU-8IT-1263SA5–7Translucent1.7
TUTVU-9IT-1263XVII5–7Translucent1.7
TUTVU-10IT-18VIII5–7Translucent1.6
TUTVU-11IT-1263XVI5–7Translucent1.8
TUTVU-12IT-18XVI5–7Translucent1.5
RuaceTUTVU29IT-1263VII500IIIDF5–7Translucent1.0+
TUTVU30IT-1263XVI750VJGG7–10Milky1.0+
TUTVU31IT-1263SA730IVKHH2–4Translucent2.3+
TUTVU32IT-18IX1.000IXLED7–10Milky1.0+
TUTVU33IT-18I258IMII2–4White2.1+
TUTVU34IT-18X1,000IXAED5–7Milky1.1+
TUTVU35IT-1263XII1,000IXGCD5–7White1.2+
TUTVU36IT-1263XII1,000IXGCD5–7Milky1.1+
TUTVU37IT-1263IXNA7–10Milky1.0+
TUTVU38IT-1263XIII1,200XINJD5–7Milky1.1+
TUTVU39IT-1263XI1,000IXOCD5–7Milky1.1+
TUTVU40IT-1263XIII500IIDDJ2–4Milky2.5+
TUTVU41IT-1263XII980VIIIGCD7–10White1.1+
TUTVU42IT-18I770VIPKD2–4White2.4+
TUTVU43IT-18XII550IIIQL-5–7White1.1+
TUTVU44IT-18XVII980VIIIEAD5–7White0.8+
TUTVU45IT-1263XVII980VIIIEAD7–10Translucent0.8+
TUTVU46IT-1263X980VIIICCD7–10Translucent0.9+
TUTVU47IT-18I550IIIQKK7–10Milky0.7+
TUTVU48IT-18III980VIIICCD7–10White0.7+
TUTVU49IT-18IIINA7–10Translucent1.0+
TUTVU50IT-18XII1,350XIIQM-2–4Translucent2.1+
TUTVU51IT-18XV1,000IXCCD5–7Milky1.0+
TUTVU52IT-1263XV1,000IXCCD5–7Milky1.0+
TUTVU53IT-1263VI1,000IXEAD5–7Milky1.0+
TUTVU54IT-1263VI500, 258XIIIRNL5–7Milky0.7+
TUTVU55IT-1263III1,000IXCCD5–7White0.8+
TUTVU56IT-1263III1,000IXCCD5–7Translucent0.9+
TUTVU57IT-1263III1,000IXCCD5–7Translucent0.8+
TUTVU58IT-18XII600, 500XIVDOE5–7Translucent1.1+
TUTVU59IT-18II500IIIDM5–7Translucent1.1+
TUTVU60IT-18III1,000IXCAD7–10White1.0+
TUTVU101IT-18IX5–7Milky1.8
TUTVU102IT-18I5–7White1.9
TUTVU103IT-18XIV5–7White1.5
TUTVU104IT-18XIV5–7White1.4
TUTVU105IT-18X5–7Milky1.7
TUTVU106IT-18X5–7Milky1.3
TUTVU107IT1263XVII5–7Milky1.4
TUTVU108IT-18XIII7–10White1.0
TUTVU109IT-18VI7–10White1.8
TUTVU110IT-18X7–10Milky1.2
SussundengaTUTVU61IT-18III1,000IXCAD5–7White1.0+
TUTVU62IT-1263III1,000IXCAD5–7White0.9+
TUTVU63IT-18SA1,000IXCAD5–7Milky1.0+
TUTVU64IT-18XIV1,000IXCAD5–7Translucent0.8+
TUTVU65IT-18VI1,000, 258XVAAN7–10Translucent0.9+
TUTVU66IT-1263XIII1,000, 500XVISAO7–10White1.1+
TUTVU67IT-1263VII1,200XITAP2–4Translucent2.4+
TUTVU68IT-18I1,200XIKPQ2–4Translucent2.3+
TUTVU69IT-18I1,000IXCAD7–10White1.0+
TUTVU70IT-1263III1,000IXCAD7–10Milky1.0+
TUTVU71IT-18II1,000IXUAQ7–10Milky1.0+
TUTVU72IT-1263III1,000IXCAD7–10Milky1.1+
TUTVU73IT-1263III1,000IXCAD7–10White1.0+
TUTVU74IT-18III1,000IXKAD7–10Translucent0.8+
TUTVU75IT-18VIII1,000IXCAD7–10Translucent0.8+
TUTVU76IT-18III1,000IXCAD5–7Milky0.8+
TUTVU77IT-1263XIV800VIIUKQ5–7Milky0.9+
TUTVU78IT-1263XIV1,000IXCAD5–7White0.9+
TUTVU79IT-18XIV1,000IXCAD5–7Milky0.8+
TUTVU80IT-1263XVI1,000IXCAD5–7White1.1+
TUTVU81IT-1263XI1,000IXCAD5–7Milky1.1+
TUTVU82IT-18XI1,000IXCAD5–7Milky1.1+
TUTVU83IT-1263XVI1,000IXCAD5–7Milky1.1+
TUTVU84IT-18X1,000IXCAD5–7Milky1.0+
TUTVU85IT-1263X1,000IXCAD5–7Milky1.0+
TUTVU86IT-1263X1,000IXCA-5–7Milky0.9+
TUTVU87IT-1263X1,000IXCAD5–7White0.9+
TUTVU88IT-18X1,000IXCAD5–7White0.9+
TUTVU89IT-1263X1,000IXCAD5–7White0.8+
TUTVU90IT-18X1,000IXCAD5–7Milky0.8+
TUTVU91IT-18X1,000IXCAD5–7Milky0.8+
TUTVU92IT-1263X500IICCR5–7Milky1.1+
TUTVU93IT-18XIII1,000IXCAD5–7Milky1.0+
TUTVU94IT-18III1,000IXCAD5–7Translucent1.1+
TUTVU95IT-18V1,000IXCAD5–7Translucent1.0+
TUTVU96IT-18III1,000IXCAD5–7Translucent1.0+
TUTVU97IT-1263III1,000IXCAD5–7Translucent1.0+
TUTVU98IT-18III600, 500XIVDQS5–7Translucent0.8+
TUTVU99IT-1263V1,000, 500XVIVRT5–7Milky0.9+
TUTVU100IT-1263V5–7White1.9
TUTVU112IT-1263IV7–10Milky1.4
InoculantBIOFIX1,000, 1,200XVIIWSU
  • a NA, not amplified. —, not determined.