Table 1.

Reactivities of serum polyclonal antibodies against culture supernatants of LAB strains as determined by NCI-ELISA, CI-ELISA, and CD-ELISA

Microorganism (bacteriocin produced)SourceaCross-reactivity (%) in:
NCI-ELISAbCI-ELISAcCD-ELISAc
Pediococcus acidilactici 347 (pediocin PA-1)Our collection100100100
Pediococcus acidilactici 347 Ped(non-pediocin PA-1 producer)Our collection1.6 NRd NR
Pediococcus pentosaceus FBB61 (pediocin A)TNONRNRNR
Enterococcus faecium T136 (enterocins A and B)Our collection2.31.91.7
Enterococcus faecium P13 (enterocin P)Our collection1.10.50.2
Enterococcus faecium L50 (enterocins L50A and L50B)Our collection1.1NRNR
Enterococcus faecalis INIA4 (enterocin AS-48)INIA1.3NRNR
Lactobacillus sake 148 (lactocin S)Our collection3.23.0NR
Lactococcus lactis BB24 (nisin A)Our collection3.53.61.9
Lactococcus lactisMG1614 (nonbacteriocin producer)IFR2.31.92.7
  • a IFR, Institute of Food Research, Norwich Laboratory (Norwich, United Kingdom); INIA, Instituto Nacional de Investigación y Tecnologı́a Agraria y Alimentaria (Madrid, Spain); TNO, Nutrition and Food Research (Zeist, The Netherlands).

  • b Cross-reactivity was defined as [(absorbance reading above the limit of detection produced by a culture supernatant/absorbance reading above the limit of detection produced by a supernatant of P. acidilactici 347) × 100].

  • c Cross-reactivity was defined as [(antibody binding inhibition produced by a culture supernatant/antibody binding inhibition produced by a supernatant of P. acidilactici 347) × 100].

  • d NR, no reactivity.