Table 5.

Specificity of gyrB PCR primers in the differentiation of B. cereus, B. thuringiensis, B. anthracis, and B. mycoides

Bacterial strain and serotypeaAmplification of a specific PCR fragmentb that identified the strain as:
B. cereusB. thuringiensisB. anthracis
B. cereus
 JCM 2152T+
 H1+
 H2+
 H3+
 H4+
 H5+
 H6+
 H7+
 H8+
 H9+
 H10+
 H11+
 H12+
 H13+
 H14+
 H15+
 H16+
 H17+
 H18+
B. thuringiensis
 berliner (IAM 12077T)+
 kurstaki (HD-1)+
 galleriae+
 aizawai+
 israelensis+
B. anthracis Pasteur#2H+
B. mycoides ATCC 6462T
  • a JCM, Japan Collection of Microorganisms; IAM, Institute of Applied Microorganisms; ATCC, American Type Culture Collection; and T, type strain.

  • b PCR primer sets BC1-BC2r, BT1-BT2r, and BA1-BA2r were used to amplify B. cereus-,B. thuringiensis-, and B. anthracis-specific fragments, respectively.