Table 2.

Fatty acid compositions of P. profundumstrains as a function of growth pressure and temperaturea

StrainCulture conditionsbMean % (by wt) of fatty acid ± SDUnsaturation indexcUFA/SFA ratiod
12:014:014:1iso-16:016:016:112-OH18:018:120:5
DB110
 Untreated15°C1.8 ± 0.94.9 ± 1.41.2 ± 0.89.8 ± 3.121.7 ± 2.841.3 ± 8.33.0 ± 1.21.1 ± 0.49.9 ± 2.65.3 ± 1.179 ± 5.11.95
4°C2.7 ± 1.03.3 ± 0.82.5 ± 0.50.2 ± 0.120.2 ± 3.848.8 ± 6.83.4 ± 1.30.2 ± 0.110.3 ± 1.78.4 ± 1.1104 ± 6.62.65
0.1 MPa1.5 ± 0.15.0 ± 0.30.8 ± 0.121.4 ± 1.564.1 ± 1.50.6 ± 0.10.2 ± 0.03.6 ± 0.12.7 ± 0.182 ± 2.42.53
28 MPa2.2 ± 0.61.6 ± 0.70.7 ± 0.117.5 ± 3.248.7 ± 9.62.0 ± 0.80.1 ± 0.116.2 ± 1.611.0 ± 0.8121 ± 3.33.57
50 MPa1.4 ± 0.80.8 ± 0.50.5 ± 0.112.5 ± 1.245.9 ± 7.02.3 ± 0.50.1 ± 0.125.1 ± 4.111.4 ± 0.2129 ± 7.25.60
 Cerulenin treatede 15°C4.9 ± 0.821.7 ± 2.74.5 ± 1.60.3 ± 0.328.3 ± 4.327.3 ± 4.23.8 ± 2.10.2 ± 0.10.3 ± 0.28.7 ± 2.676 ± 8.90.79
4°C9.3 ± 0.017.9 ± 0.89.8 ± 2.319.0 ± 0.422.4 ± 0.68.3 ± 0.10.3 ± 0.113.0 ± 0.998 ± 2.70.98
0.1 MPa9.5 ± 0.324.8 ± 3.25.6 ± 1.030.0 ± 2.910.8 ± 0.86.2 ± 0.70.2 ± 0.00.3 ± 0.012.6 ± 1.180 ± 1.90.45
28 MPa16.1 ± 2.335.1 ± 3.75.3 ± 0.917.2 ± 2.07.1 ± 1.16.3 ± 0.912.9 ± 1.277 ± 1.70.37
EA3
15°C8.2 ± 1.319.7 ± 2.311.7 ± 2.821.5 ± 1.427.9 ± 2.95.0 ± 2.63.5 ± 0.50.9 ± 0.51.6 ± 0.349 ± 6.60.80
4°C9.2 ± 1.224.0 ± 0.610.9 ± 1.222.0 ± 0.720.9 ± 1.94.3 ± 1.60.3 ± 0.10.1 ± 0.08.3 ± 0.673 ± 3.90.72
0.1 MPa3.9 ± 0.219.3 ± 1.73.6 ± 0.253.4 ± 0.511.9 ± 1.13.5 ± 0.20.8 ± 0.10.5 ± 0.13.1 ± 0.232 ± 2.60.25
28 MPa3.6 ± 0.117.9 ± 0.43.1 ± 0.153.2 ± 0.115.3 ± 0.62.7 ± 0.31.2 ± 0.10.7 ± 0.12.3 ± 0.231 ± 0.50.28
EA2
15°C3.5 ± 0.316.5 ± 1.21.1 ± 0.135.9 ± 0.510.1 ± 0.33.7 ± 2.02.6 ± 0.70.7 ± 0.125.8 ± 1.2141 ± 6.00.64
4°C4.9 ± 0.224.4 ± 3.21.7 ± 0.626.9 ± 1.07.1 ± 0.54.7 ± 1.50.4 ± 0.10.2 ± 0.129.5 ± 1.9157 ± 3.60.68
0.1 MPa4.5 ± 0.125.2 ± 0.10.5 ± 0.535.8 ± 2.23.9 ± 0.14.5 ± 1.10.4 ± 0.20.2 ± 0.124.8 ± 0.9129 ± 4.50.45
28 MPa4.3 ± 0.615.5 ± 3.11.8 ± 0.329.7 ± 7.09.1 ± 0.13.9 ± 0.90.2 ± 0.10.8 ± 0.432.7 ± 4.5176 ± 21.00.89
50 MPa4.0 ± 0.15.0 ± 0.10.4 ± 0.135.6 ± 1.318.3 ± 1.05.1 ± 0.60.3 ± 0.02.8 ± 0.128.3 ± 2.7163 ± 12.41.11
EA10
15°C2.5 ± 1.06.5 ± 2.24.7 ± 0.55.0 ± 1.516.7 ± 2.846.3 ± 3.83.3 ± 0.71.0 ± 0.214.0 ± 1.465 ± 3.02.43
4°C3.0 ± 0.23.2 ± 0.74.1 ± 0.72.1 ± 1.010.6 ± 0.955.2 ± 3.93.9 ± 0.20.2 ± 0.117.7 ± 3.377 ± 1.34.53
0.1 MPa3.1 ± 0.13.7 ± 0.32.2 ± 0.320.4 ± 0.552.9 ± 1.04.6 ± 0.40.4 ± 0.112.7 ± 0.168 ± 0.82.46
28 MPa2.8 ± 0.21.4 ± 0.21.6 ± 0.012.2 ± 2.052.3 ± 0.34.2 ± 0.10.1 ± 0.125.4 ± 2.679 ± 2.44.81
50 MPa2.6 ± 1.01.6 ± 0.31.1 ± 0.213.5 ± 0.351.6 ± 3.65.5 ± 1.20.1 ± 0.124.0 ± 3.877 ± 0.24.31
  • a Data represent values derived from triplicate samples harvested in the late exponential phase of growth.

  • b 15 and 4°C cultures were grown aerobically in 2216 marine medium at 0.1 MPa; 0.1-, 28-, and 50-MPa cultures were grown at 9°C in 2216 marine medium containing 22 mM glucose buffered with 100 mM HEPES.

  • c Unsaturation index was calculated as the sum of the mean percentages (by weight) of the UFA species multiplied by the number of double bonds (12).

  • d UFAs, 14:1, 16:1, 18:1, and 20:5; SFAs, 12:0, 14:0, 16:0, and 18:0.

  • e Cerulenin was used at 12 μg/ml.