Table 4.

gyrB PCR primers in the differentiation ofB. cereus, B. thuringiensis, B. anthracis, and B. mycoides

SpecificityPrimerOligonucleotide sequenceNo. of bpa
Universal primersbUP-1GAA GTC ATC ATG ACC GTT CTG CAY GCN GGN GGN AAR TTY GA41
UP-2rAGC AGG ATA CGG ATG TGC GAG CCR TCN ACR TCN GCR TCN GTC AT44
B. cereusBC1ATT GGT GAC ACC GAT CAA ACA21
BC2rTCA TAC GTA TGG ATG TTA TTC21
B. thuringiensisBT1ATC GGT GAT ACA GAT AAG ACT21
BT2rCCT TCA TAC GTA TGA ATA TTA TTT24
B. anthracisBA1AAT CGT AAT ATT AAA CTG ACG21
BA2rCCT TCA TAC GTG TGA ATG TTG21
  • a bp, base pairs.

  • b Universal primers were as described by Yamamoto and Harayama (50).