Table 1.

Bacterial strains used in this study and results of PCR amplification of cd 1- and Cu-nirgenes and Southern blot hybridization of PCR products

Bacterial strainSource or referenceaNir typeb (reference)Result of:
PCRc with nirprimersHybridizationd withnir probes
cd1Cucd1Cu
Denitrifying strains
 Pseudomonas stutzeri ATCC 14405ATCCcd (13)+0+
 P. stutzeri CCUG 29240CCUG?++
 P. fluorescens ATCC 33512ATCCcd (5)+
 P. fluorescensMi32SLU?++
 P. aeruginosaCCUG 241CCUG?+0+
 P. aeruginosa Mi11SLU?++
 P. putida CCUG 2479CCUG?0+
 P. aureofaciens ATCC 13985ATCCCu (5)
 P. denitrificansATCC 13867CCUGCu (5)0++
 P. denitrificans CCUG 2519CCUG?0
 Paracoccus denitrificans ATCC 19367CCUGcd (5)0
 P. denitrificansPd1222A. H. Stouthamercd (6)0++
 Ralstonia eutropha e CCUG 13724CCUG?++
 Alcaligenes eutrophus ATCC 17699ATCCcd (24) or Cu (31)00
 A. faecalis ATCC 8750ATCC?0++
 A. faecalis ATCC 19018CCUG?0+
 A. denitrificansATCC 15173CCUG?
 Achromobacter cycloclastes ATCC 21921ATCCCu (5)++
Nondenitrifying strains
 Escherichia coliTG122?
 Staphylococcus aureus8325-418?
  • a ATCC, American Type Culture Collection; CCUG, Culture Collection of the University of Gothenburg; SLU, Swedish University of Agricultural Sciences (U. Granhall); A. H. Stouthamer, Vrije Universiteit, Amsterdam, Amsterdam, The Netherlands.

  • b ?, no data found in the literature.

  • c +, visible band of the expected size; 0, band of any other size; −, no visible band.

  • d A positive hybridization signal (+) was defined as a visible band of the expected size (45°C).

  • e Formerly known as A. eutrophus.