Table 1.

Effects of different carbon sources and carbon source depletion on ech42 expression in T. atroviride

Carbon sourceConcn (% [wt/vol])Meanech42 expression (SD)a
GOX formed (mg/mg [dry wt])GOX activity (U/mg [dry wt])
Glucose0.13.7 (0.7)4.4 (0.48)
1<0.10.07 (0.04)
Glucose + chitin0.1 + 0.54.3 (1.3)3.9 (0.50)
1 + 0.5<0.10.05 (0.04)
Glycerol0.11.8 (0.3)3.1 (0.47)
1<0.10.14 (0.005)
Glycerol + chitin0.1 + 0.52.1 (0.4)2.0 (0.44)
1 + 0.5<0.10.09 (0.01)
  • a All values shown are for 48 h of cultivation and are from at least three separate experiments with allech42::gox transformants described in Fig. 1 and normalized to a single gene copy. Values for 24 h were below the detection limit in all cases and are not given. GOX, glucose oxidase.