Table 2.

Oligonucleotides used in this work

PrimerSequence 5′→3′a
PPK5RGTTGCGGATCATCCAGTC(C/G)GC
PPK2FGT(G/C)CGCTTCGC(G/C)GA(G/A)CT(G/C)AA(G/A)
PPK5FGGTATCGCGGCGGTACCGGATC*TCAACGAGACCC
Tc1GATCAGATCTGAATAAGGGCGACACGGAAA
Tc2GATCAGATCTCTCAACGACAGGAGCACGAT
K1TCCTTGCATGCCCTACC
K2CGGGGTACCTCAATGATGATGATGATGATGACGTGCGGTAAGC
PPK-NCGGAATTCGTGGATGACAGC
PPK-CCCCAAGCTTACGTGCGGT
PPX-NCGGAATTCATATGGACTTGC
PPX-CCCCAAGCTTATTACCGCACGTTG
P1CACGCGGATGTTGAACTGCAACTG
  • a Underlined sequences are complementary to sequences deposited in GenBank. An asterisk (*) indicates a missing C to eliminate a BamHI site.