Table 3.

Glycine betaine and choline uptake activitiesa

SpeciesUptake (nmol/min/mg of protein)b
Glycine betaineCholine
−NaCl+NaCl+GB−NaCl+NaCl
R. leguminosarum bv. viciae0.030.55 (50)0.920.270.06
R. leguminosarum bv. trifolii0.160.50 (50)1.340.950.23
R. leguminosarum bv. phaseoli0.10 NGc (50)NDd 0.60NG
R. tropici IIA0.240.14 (100)2.190.320.27
R. tropici IIB0.330.24 (150)1.671.580.90
A. rhizogenes 0.06NG (50)ND 0.42NG
S. meliloti 1.533.17 (150)3.572.750.21
S. fredii 0.460.04 (150)3.670.760.10
R. etli 0.060.09 (50)1.800.420.03
R. galegae 0.080.02 (150)1.880.340.21
A. tumefaciens 0.130.22 (150)1.963.451.27
M. loti 0.120.02 (100)ND 0.220.09
M. huakuii 0.280.52 (100)ND 1.273.03
B. japonicum <0.01NG (50)<0.01<0.01NG
  • a Cells were grown to late log phase in LAS medium without added NaCl (−NaCl), with the salt added (+NaCl) (at the concentration [millimolar] given in parentheses), or without salt in the presence of 5 mM glycine betaine (+GB).

  • b Transport activities were measured at a concentration of 1 μM. The values obtained with triplicate independent cultures never varied more than 10%.

  • c NG, no growth.

  • d ND, not determined.