Table 1.

Summary of CGTase purification steps

FractionActivitya (U)Amt of protein (mg)Sp act (U/mg)Purification (fold)Yield (%)
Crude sample95.8123.50.81.0100
Resource Q59.512.84.65.862
Phenyl-Superose15.81.6809.411.816
α-CD affinity9.80.0071,4001,75010
  • a CD synthesis activity was measured at 90°C.