Table 4.

CDH and BADH activities from different rhizobiaa

SpeciesEnzyme activity (nmol/min/mg of proteins)b
CDHBADH
R. leguminosarum bv. viciae33103
R. leguminosarum bv. trifolii793
R. leguminosarum bv. phaseoli16134
R. tropiciIIA2828
R. tropici IIB3941
A. rhizogenes 1283
S. meliloti 33126
S. fredii 1292
R. etli 118
R. galegae 841
A. tumefaciens 2626
M. loti 2253
M. huakuii <1<1
B. japonicum <1<1
  • a Cells were grown in LAS medium supplemented with 7 mM choline.

  • b Toluene-treated cells and cells disrupted by passage through a French press were used for CDH and BADH assays, respectively. See Materials and Methods for details. Data are the means of results from duplicate assays with two independent cultures (standard deviations never exceeded 9%).