Table 2.

Equilibrium metal speciation of media during measurement of whole-cell sMMO activity

Metal speciationResult in medium (chelating agent)
NMS (EDTA)M2M (pyrophosphate)M2M (EDTA)
Total Cu (μM) 0–0.13 0–0.080.01–0.2
Chelated Cu (%)97.799.9100
Precipitated Cu (%)2.200
Free Cu (%)0.150.06<10−4
pCua10.3–9.8 10.8–10.3>11.6
Total Fe (μM)9.41.21.0
Soluble Fe (%)95820
Precipitated Fe (%)59280
  • a Free copper concentrations at equilibrium, reported as the negative log of [Cu2+].