Table 1.

Environments sampled for members of candidate division WS6

EnvironmentaAnnealing temp attempted (°C) PCR product obtainedbLibrary annealing temp (°C)Library nameNo. of clones
AnalyzedUnique
Hot spring cyanobacterial mat (YNP)50, 55, 60++551A482
Berkeley marina sediment (Calif.)45, 50, 55, 60, 65++60BMS19611
Berkeley marina sediment (Calif.)55c +55BMS963
Bolinas sediment (Calif.)45, 50, 55, 60, 65++55BOL12820
Hydrocarbon-contaminated aquifer (Calif.)45, 50, 55, 60, 65++55CA112811
Hydrocarbon-contaminated aquifer (Mich.)d 50++50WCHB1923
Obsidian Pool hot spring sediment (YNP)50, 55, 60+55OP41
Lake Lemon (Ind.) sediment45, 50, 55, 60, 65++60LL328
Landscaped topsoil (Berkeley)50, 55, 60++55D1961
Pavilion Lake (Canada) microbialite55, 60
Fairfax Swamp (Ind.) sediment sample55, 60
2 Human fecal samples50, 55, 60
4 Human intestine samples50, 55, 60
Queen's Laundry hot spring orange sediment (YNP)50, 55, 60
  • a YNP, Yellowstone National Park.

  • b ++, product after one round of PCR; +, product after two rounds of PCR; −, no PCR product detected.

  • c WS6-specific primer not used in amplification.

  • d Original study.