Table 2.

Diversity indices calculated from LH-PCR and FAME data

MethodSiteRichnessaEvennessbDiversityc
LH-PCRA20.0 ± 0.0cd 0.91 ± 0.01b2.73 ± 0.02c
B19.3 ± 0.5d0.90 ± 0.01c2.65 ± 0.04d
C122.0 ± 0.0b0.91 ± 0.01b2.80 ± 0.02b
C223.0 ± 0.0a0.92 ± 0.00a2.90 ± 0.01a
FAMEA34.0 ± 0.8a0.85 ± 0.00a3.00 ± 0.02a
B30.5 ± 2.9b0.83 ± 0.02b2.82 ± 0.09b
C131.8 ± 1.0ab0.86 ± 0.00a2.96 ± 0.02a
C232.4 ± 1.0ab0.85 ± 0.01a2.96 ± 0.02a
  • a Richness is equal to the number of LH-PCR or FAME peaks.

  • b Evenness is equal to diversity/ln (richness).

  • c Diversity (Shannon index) (H) was calculated as follows: H = −Σpiln (pi), where pi is the relative abundance of a given LH-PCR or FAME peak.

  • d Values are means ± standard deviations (n = 4). For each index, different letters indicate that values for different sites determined by the same method were significantly different (P < 0.05), as determined by one-way analysis of variance and least significant difference.