Table 1.

Identities and frequencies of occurrence (on six sampling dates) of the 18S rDNA clones in the second stages of our systems

Clone(s)aTaxonPanel in Fig. 3Frequency (no. of dates)
Alga 1Alga 2Cyano 1Cyano 2
LKM67CiliophoraA5441
LKM48RhizopodaC1000
LKM62, LKM63b CiliophoraA1002
LKM43CiliophoraA5456
LKM8CiliophoraA3344
LKM45RhizopodaC2446
LKM88MetazoaD3034
LKM74AmoebaeB3411
LKM30RhizopodaC2403
LKM36CiliophoraA4200
LKM15LKM11c B2211
LKM51DRIPsd D0313
LKM33FungiD3013
LKM11LKM11c B2320
LKM46LKM11c B3010
LKM80MetazoaD1001
LKM85Metazoae 1234
LKM101KinetoplastidaE1323
LKM118Unknown0100
  • a The clones are arranged in order of their melting positions on the DGGE gel, beginning at the top of the gel (Fig. 2).

  • b Clones LKM62 and LKM63 produced the same DGGE band in a 30 to 55% denaturing gradient.

  • c LKM11, LKM15, and LKM46 form a unique terminal clade whose affiliation is not known; this clade was provisionally named after clone LKM11.

  • d The DRIPs phylogenetic clade currently comprises the following taxa: Dermocystidium salmonis,Dermocystidium sp., rosette agent (unknown L29455 sequence),Ichtypphonus hoferi, Psorospermium haeckelii(26), and a sequence from a protozoan formerly classified as the unpigmented unicellular alga “P. richardsi” (5).

  • e The sequence of clone LKM85 was incomplete (S. cerevisiae sequence positions 4 to 558 and 630 to 1643) and therefore was not included in the phylogenetic analysis shown in Fig. 3D. The overall level of similarity of LKM85 to Brachionus plicatilis was 99.4%.