Table 1.

Bacterial strains and plasmids used in these studies

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
P. putida
  GPp104PHA synthase-negative mutant of P. putida KT2442 (mt-2,hsdR1 [r m+]) without TOL plasmid 5
  PHAGN-21PhaG-negative mutant of P. putida KT2440 15
P. fragi Wild typeDSM 3456
P. oleovorans OCT plasmidATCC 29347
E. coli
  S17-1 proA thi-1 recA; harbors the tragenes of plasmid RP4 in the chromosome 19
  JM109 recA1 endA1 gyrA96 thi-1 hsdR17(rk, mk+) supE44 relA1 λ lac [F′ proAB lacI qZΔM15] 17
  RS3097 e14 (mcrA ) fadR41(ts)zcg-101::Tn10 20
Plasmids
 pBHR71pBluescript SK(−) containingphaC1 from P. aeruginosa downstream oflac promoter 8
 pBHR75pUCP27 containing the 1.3-kbp BamHI-HindIII fragment comprising phaG, including the native promoter 15
 pBBR1MCS-2Kmr, broad host range, lacPOZ 7
 pBHR81pBBR1MCS-2 containing coding region of phaGdownstream of lac promoter 15
 pSF2pBBR1MCS-2 containing coding region of phaC1gene from P. aeruginosa downstream of lacpromoterThis study
 pJRDEE32pJRD215 containing thephaC and phaJ genes of A. caviae 3
 pPS2pBBR1MCS-2 containing coding region of phaC gene from A. caviaedownstream of lac promoterThis study
 pBHR86pBBR1MCS-2 containing coding region of phaC1gene from P. aeruginosa downstream of lacpromoter and coding region of phaG from P. putidadownstream of phaC1, including the native promoterThis study