Table 3.

Accumulation of PHA in recombinant P. fragivia β-oxidation pathwaya

PlasmidCarbon sourceAcrylic acid concn (mg/ml)PHA content (% [wt/wt] of CDW)Composition of PHA (mol%)
3HB3HHx3HO3HD3HDD3HDD:1
pBBR1MCS-2Decanoate0.0Traceb NDNDNDTraceTraceND
0.1TraceNDNDNDTraceTraceND
0.2TraceNDNDNDTraceTraceND
pBBR1MCS-2Gluconate0.0TraceNDNDNDTraceTraceND
0.1TraceNDNDNDTraceTraceND
0.2TraceNDNDNDTraceTraceND
pSF2Decanoate0.0TraceNDNDTraceTraceTraceND
0.12.8ND 36136TraceND
0.23.3NDND4258TraceND
pPS2Decanoate0.0TraceTraceTraceNDTraceTraceND
0.12.26535NDTraceTraceND
0.25.57129NDTraceTraceND
pBHR86Decanoate0.0TraceNDNDTraceTraceTraceND
0.13.7ND 35038 9ND
0.26.8ND 23459 5ND
pBHR86Gluconate0.010.0ND 11669104
0.17.4ND 21664135
0.2NG
  • a Cells were cultivated on LB medium containing 0.2% (wt/vol) decanoate or 1.5% (wt/vol) sodium gluconate. Acrylic acid was applied to inhibit β-oxidation. Cultivations were performed for 48 h at 30°C. Abbreviations: 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate; 3HDD:1, 3-hydroxydodecenoate; ND, not detectable.

  • b Trace, ≤1% of CDW.