Table 2.

Locations and sizes of ORFs in pTfars1b

ORFPositionNo. of amino acidsSize (kDa)Closest relationship to a known proteinaProduct detectedb
ORF 11014-NDc 338ND41% identity and 57% similarity to putative membrane protein of E. coli (accession no. ACC73882)Yes
arsC 1752-126116318.153% identity and 65% similarity to P. aeruginosa ArsC (accession no. AAC69644)Yes
arsR 1752-210811813.135% identity and 47% similarity to Mycobacterium tuberculosis hypothetical protein Rv2640c (accession no. CAB02346)Yes
arsB 2202-351243648.457% identity and 70% similarity to ArsB from E. coli plasmid R773 (accession no. P08691)Yes
arsH 3518-424024026.768% identity and 81% similarity to Y. enterocolitica ArsH (accession no. AAB42206)Yes
ORF 54348-572445844.758% identity and 72% similarity to signal recognition particle protein (fifty-four homologue) (P48) of E. coli (accession no. BAA16495)Yes
ORF 65795-6055869.5666% identity and 79% similarity to 30S ribosomal protein S16 of Haemophilus influenzae Rd KW20 (accession no. P44382)No
ORF 76111-655414712.644% identity and 58% similarity to 16S rRNA-processing protein RIMM of Haemophilus influenzaeRd KW20 (accession no. P44568)No
ORF 86554-ND94ND34% identity and 52% similarity to tRNA (guanine-N1)-methyltransferase of Salmonella typhimurium (accession no. P36245)No
  • a The identity and similarity values are based on amino acid sequence data. The accession numbers are GenBank-EMBL database accession numbers.

  • b Protein products were detected by using an in vitro E. coli transcription-translation system.

  • c ND, not determined. The ORF was incomplete, and the molecular mass was not calculated; however, the number of amino acids is indicated.