Table 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionaReference or source
E. coli
 S17.1recA pro thi hsdR RP4-2-Tc::Mu-Km::Tn7Tra+ TprSmr50
 HB101recA13 proA leuB6 hsdR rpsL20(Smr)13
 CC118λpirΔ(ara-leu) araDE ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1 λpir; phage lysogen29
 DH5αsupE44 thi-1 recA1 hsdR17 endA1 gyrA(Nalr) Δ(lacIZYA-argF)U169 deoR[φ80dlacΔ(lacZ)M15]27
Pseudomonas sp. strain M1Myr+This study
P. putida NCIMB 8248NCIMB wild-type strainb
Pseudomonas sp. strain N22Myr mutant obtained by transposon mutagenesisThis study
pRK2013RK2-Tra+RK2-Mob+ KmroriColE122
pUT mini-Tn5KmApr Kmr; ori R6K RP4oriT delivery plasmid for mini-Tn5Km20
pBluescript KS(+)Aprα-lac/MCSStratagene
pLAFR3Broad-host-range cosmid vector IncP1 RK2 replicon; λcos+lacZα Mob+ TraTcr9
pC7Tcr; pLAFR3 with a 22-kb chromosomal fragment from Pseudomonassp. strain M1This study
pB22ScAprKmr; pBluescript KS(+) with the 7.7-kb SacI fragment containing mini-Tn5This study
pB2.3 BgScApr; pBluescript KS(+) with the 2.3-kbBglII-SacI fragment from pB22ScThis study
pB5.3 ScApr; pBluescript KS(+) with the 5.3-kbSacI fragment from pC7This study
  • a Myr, myrcene-degradative phenotype; Apr, ampicillin resistance; Kmr, kanamycin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance; MCS, multiple cloning site.

  • b NCIMB, National Collections of Industrial and Marine Bacteria, Aberdeen, Scotland, United Kingdom.