Table 1.

Effects of carbon source (glucose-acetate or diesel fuel) and temperature (5 or 24°C) on lectin binding to cells and exopolymer of Rhodococcus sp. strain Q15

Lectin-fluor conjugateaGlycoconjugate(s)Growth at 5°C on:Growth at 24°C on:
Glucose-acetateDiesel fuelGlucose-acetateDiesel fuel
Arachis hypogaea-TRITCβ-Gal(1->3)-GalNAcNo bindingBinding to cell surface and surfaces of diesel fuel microdropletsNo bindingNo binding
Canavalia ensiformisA-FITCα-Glucose, α-mannoseWeak cell surface bindingBinding to cell surface and surfaces of diesel fuel microdropletsStrong binding to exopolymerStrong binding to exopolymer
Tetragonolobus purpureas-TRITCα-l-FucoseWeak cell surface bindingBinding to cell surfaceWeak cell surface bindingNo binding
Triticum vulgaris-TRITC(GlcNAc)2, NeuNAcVery weak cell surface bindingWeak binding to cells and exopolymerWeak binding to cells and exopolymerWeak cell surface binding
Ulex europeaus-TRITCα-l-Fucose, (GlcNAc)2 No bindingBinding to cell surfaceStrong binding to exopolymerNo binding
  • a TRITC, tetramethyl rhodamine isothiocyanate; FITC, fluorescein isothiocyanate.