Table 3.

DNA isolation comparisonsa

StrainProcedureA260/A280DNA concn (μg/μl)
S. badius252Marmur0.890.246
5′→3′1.200.717
Rao1.831.68
Heath1.872.98
S. viridosporusT7AMarmur1.120.342
5′→3′1.480.951
Rao1.925.40
Heath1.840.500
Streptomyces sp. strain WYE 27Marmur1.281.077
5′→3′1.150.426
Rao1.904.14
Heath1.543.71
Streptomyces sp. strain YCED 5Marmur1.090.291
5′→3′1.010.327
Heath1.900.97
  • a Cell pellets were normalized on all small-scale DNA-extracted samples to 0.2-ml centrifuged pellet volume.