Table 1.

Bacterial strains, phage, plasmids, and primers used in the present study

MaterialRelevant featuresSource or reference
Bacteria
E. coliDH10BUsed for general cloningGIBCO-BRL
L. casei ATCC 393Plasmid-freeATCCa
Plasmids
 pUC19EpUC19 derivative, Emr18
 pBT338pUC18::six29
 pLP825p8014-2, ori of pBR322, Cmr25
 pBT241pT712-borne β gene29
 pEM40pUC19E-borne A2int-attP3
 pEM40::cIpUC19E-borne A2 cI,int-attP3
 pEM74pUC19E-bornesix, A2 int-attPThis work
 pEM76pUC19E-borne six1 A2 int-attP six2This work
 pEM76::cIpUC19E-borne six1 A2cI int-attP six2This work
 pEM68pLP825-borne β geneThis work
Phage A2Temperate phage isolated from milk whey13
Primers
 att15′-AGCAGGACGAGAAAGCAATGAATGT-3′3
 att75′-GCCGGTGTGGCGGAATTGGCAG-3′3
 b15′-TCTCTGATAGACAGTATAGAGGAG-3′3
 int25′-CTGGGATCCCCAAGGCTTACTTT-3′This work
  • a ATCC, American Type Culture Collection.